High-performance liquid chromatography

(HPLC); versatile chromatography method for the high-resolution separation of compounds

Description
HPLC methods are a widely applicable point of reference for the diagnosis and analysis of hemoglobin variants. HPLC systems and specialised reagent kits dedicated to hemoglobin analysis can be purchased pre-configured, facilitating comparisons across laboratories. Specifically, ion-exchange HPLC has been established as a routine clinical method for the quantification of hemoglobin fractions, while reverse-phase (RP) HPLC allows the identification and quantification of hemoglobin chains and chain variants.