Ligase chain reaction

a technique for target amplification of DNA in which DNA ligase is used to join two complementary oligonucleotide probes that have bound to a target sequence in vitro

Description
The ligation product is used as a template for annealing of complementary oligonucleotides that, through repeated enzymatic processing, allow for logarithmic accumulation of products that can be used to determine the presence of the target of interest.